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| Santiago, Christine D.; Yagi, Shogo; Ijima, Motoaki; Nashimoto, Tomoya; Sawada, Maki; Ikeda, Seishi; Asano, Kenji; Orikasa, Yoshitake; Ohwada, Takuji. |
| The compatibility of strains is crucial for formulating bioinoculants that promote plant growth. We herein assessed the compatibility of four potential bioinoculants isolated from potato roots and tubers (Sphingomonas sp. T168, Streptomyces sp. R170, Streptomyces sp. R181, and Methylibium sp. R182) that were co-inoculated in order to improve plant growth. We screened these strains using biochemical tests, and the results obtained showed that R170 had the highest potential as a bioinoculant, as indicated by its significant ability to produce plant growth-promoting substances, its higher tolerance against NaCl (2%) and AlCl3 (0.01%), and growth in a wider range of pH values (5.0-10.0) than the other three strains. Therefore, the compatibility of R170 with... |
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Palavras-chave: Plant growth-promoting bacteria; Plant tissue localization; Combined inoculation; Bacterial compatibility. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4466 |
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| 折笠, 善丈; 佐藤, 遥子; 大和田, 琢二; Orikasa, Yoshitake; Sato, Haruko; Ohwada, Takuji. |
| チーズホエイを原料に用いた焼酎製造法を検討するため，カタボライト抑制解除株 Kluyveromyces marxianus KD-15 を用いて，チーズホエイを加えた糖化同時発酵におけるエタノール生産量を改良した。Aspergillus luchuensis あるいは A. oryzae で作成された麹と酵母 3 株（K. marxianus NBRC1963（親株）と K. marxianus KD-15, Saccharomyces cerevisiae NBRC10515）を組み合わせ，培養混合物中の pH，グルコースとラクトース含量，α アミラーゼと糖化力，並びにエタノール含量を経時的に21日間測定した。その結果，K. marxianus KD-15はいずれの麹との組み合わせでもエタノール生産量が他の酵母株よりも有意に高く，A. luchuensis との組み合わせでは，親株，及び S. cerevisiae NBRC10515 より約30%向上した。α-アミラーゼ，および糖化力は培養とともに低下傾向を示したが，K. marxianus KD-15 はいずれの麹との組み合わせでもラクトース消費量が最も高かったことから，K. marxianus KD-15 による高いエタノール生産は，デンプン由来のグルコースよりもチーズホエイ由来のラクトース消費量の影響を受けると考えられた。以上の結果から，K. marxianus KD-15 はデンプン質の混合原料の存在下でも高いチーズホエイの資化とエタノール生成能を有し，チーズホエイを原料とした焼酎製造への有用性が示された。 |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4371 |
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| Mikumo, Dai; Takaya, Masahiro; Orikasa, Yoshitake; Ohwada, Takuji; 折笠, 善丈; 大和田, 琢二. |
| The wild yeast Saccharomyces cerevisiae AK46 was isolated from cherry fruits and commercialized as baker's yeast. We isolated a 2-deoxyglucose (2-DOG)-resistant mutant of AK46 (MCD4) and evaluated its leavening ability in bread dough. Our findings show that the maltose utilization of MCD4 was significantly enhanced in the presence of glucose and α-glucosidase activity was increased by approx. 1.6-fold compared to that of AK46, indicating release from catabolite repression. In addition, baking performance results using the sponge dough and no-time dough methods showed that the CO2 production rate of MCD4 was increased by approx. 1.6-fold (sponge and dough method) and the specific volume of bread was increased by 6.3% and 7.8%, respectively. These results... |
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Palavras-chave: Bread dough; Saccharomyces cerevisiae; 2-deoxyglucose; AK46; Leavening. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4368 |
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| Takeshima, Keisuke; Hidaka, Tatsuo; Min Wei2); Yokoyama, Tadashi; Minamisawa, Kiwamu; Mitsui, Hisayuki; Itakura, Manabu; Kaneko, Takakazu; Tabata, Satoshi; Saeki, Kazuhiko; Oomori, Hirofumi; Tajima, Shigeyuki; Uchiumi, Toshiki; Abe, Mikiko; Tokuji, Yoshihiko; Ohwada, Takuji; 得字, 圭彦; 大和田, 琢二. |
| The early molecular dialogue between soybean and the bacterium Bradyrhizobium japonicum is crucial for triggering their symbiotic interaction. Here we found a single large genomic locus that is widely separated from the symbiosis island and was conspicuously induced within minutes after the addition of genistein. This locus (named BjG30) contains genes for the multidrug efflux pump, TetR family transcriptional regulator, and polyhydroxybutyrate (PHB) metabolism. The induction of BjG30 by genistein was competitively inhibited by daidzein, although both genistein and daidzein are soybean-derived inducers of nodulation (nod) genes. Such a differential expression pattern is also observed in some legume-derived flavonoids, which structurally differ in the... |
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Palavras-chave: Bradyrhizobium; Genistein; Multidrug efflux pump; TetR family transcriptional regulator. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3945 |
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